Skin Project: Triple staining
The staining combination includes anti-Plakoglobin to identify all keratinocytes and Ki67 to analyse the proliferative activity of all cells. That means that Plakoglobin+/Ki67+ cells are proliferative keratinocytes and Plakoglobin-/Ki67+ cells are proliferative non-epithelial cells.
Tissue staining protocol: DAPI, Plakoglobin-Alexa488, Ki67-APC or IgG1-APC
Negative control
Positive sample
Statistical results are displayed when cut-off or gates are set. The parameters include mean intensity, relative/absolute events, and percentage of reactive cells as well as sums. The quadrant statistics (UL-Upper Left, UR-Upper Right, LL-Lower Left, LR-Lower Right) are presented in tabular format.
Gating
By the use of gates the analysis is restricted to subpopulations with defined phenotype.
Cell subpopulations can be visualised in distinctive colours by gating.
Gates can be used as input gates for new scattergrams.